Modern techniques of protein identification and Molecular Recognition Methods

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Teacher: Blasco Morozzo della Rocca

Aims:The objective of the course is the learning of basics of spectroscopies applied to Molecular recognition, as well as the uses, characteristics and limitations of the most widely used techniques
use to assess the presence of proteins and their interactions.
Microscopy based methods, single molecule manipulation and more recent methods to study protein-protein interactions and structural characterization of macromolecules.

Program: Fundamentals of light- matter interaction. Electromagnetic spectrum. Structure of matter.
Absorption and luminescence phenomena. Absorption and fluorescence spectroscopy of
biological samples. Polarization of light. Linear and circular dichroism. Fluorescence
anisotropy. Infrared and Raman (SERS) spectroscopy.
Microscopy: Principles of optics and microscopy. Optical and confocal microscopy.
Ultraresolution methods, Probe scanning microscopy (STM, AFM, SNOM).
Classic experimental methods: Chromatography, Coimmunoprecipitation, Cross linking,
Two-hybrid assay, in yeast and bacteria. Fluorescent based methods, fragment
Advanced experimental methods: Surface plasmon resonance (SPR), Atomic force
microscopy, Optical Tweezers, In vivo imaging (FRET, STED)
Overview of Computational Methods: Structural Modeling, Molecular Dynamics, Docking.

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