Teacher: Daniela Barilà
Aims: The aim of the course is to provide students with the tools for understanding and drawing experimental strategies for the study of problems related to the regulation of gene expression in eukaryotic cells useful for the development of biotechnological projects
Program: References and insights on molecular mechanism that ensure the control of gene expression: epigenetic control, transcriptional and post-transcriptional control. Role of microRNA and long non coding RNA. Translational and post-translational control. Post-translational modifications and signal transduction: positive and negative feedback loop, redundancy and robustness. Examples of technical strategies employed to study gene expression. Examples of gene expression regulation: cell cycle regulation, control of cell proliferation, genomic stability maintenance. Cancer as an example of gene expression deregulation: tumorigenesis, oncogenes and tumor suppressors, mutations. Analysis of signal transduction pathways to define novel therapeutic targets in cancer.